During a George Mason University laboratory rotation, I evaluated the efficacy of selected chemical compounds capable of activating human 2-C-Methyl-D-erythritol-4-phosphate cytidyltransferase, or IspD, in vitro. This work involved detailed colorimetric assay analysis, use of reagents such as ribitol, pyrophosphatase, malachite green dye, and ammonium molybdate, as well as the creation of standard curves to assess activity and experimentally determine enzyme-substrate productivity.